D545 (12mm) OxyProbe II Dissolved Oxygen Sensor

B2–LS104–020–M30

B5-13202-5T7

B5-11005-07-J

B2-LS103-015-M30

B4-21006-020-M10

B4-35005-020-M10

B4-41001-2T9-M10

B4-21005-015-M10

B4-35004-015-M10

B4-31024-8T9-B06

B4-31004-8T9-B06

B4-32023-5T7-B03

B4-21004-007-M10

B4-35002-005-M10

B4-31003-5T7-B06

B4-53008-020-M10

B4-22005-020-M10

B4-12004-020-M10

B2-LS202-020-M69

B5-11315-020

B5-11007-20-R

B4-82004-8T9-M27

B4-53007-015-M10

B4-22004-015-M10

B4-12003-015-M10

B4-44002-8T9-M27

B4-42002-8T9-M27

B4-72003-8T9-M27

B4-32005-8T9-B03

B4-32004-8T9-B03

B2-LS201-015-M69

B5-11314-015

B5-11006-15-R

B5-13103-8T9

B5-12103-8T9

B5-11355-07J

B4-21002-003-M10

B4-31002-2T3-B06

B4-35001-2T3-M06

B4-53006-007-M10

B4-22003-007-M10

B4-12002-007-M10

B4-32003-5T7-B03

B2-LS102-007-M30

B5-11313-007

B5-11004-07-R

B4-22002-005-M10

B5-11312-005

B5-11003-05-R

B5-13102-5T7

B5-12102-5T7

B5-11321-03J

B5-11002-03-J

B5-13201-003

B4-82002-2T7-G34

B4-82001-2T7-M18

B4-81003-2T9-M12

B4-81002-2T9-M10

B4-81001-2T3-M06

B5-51001-2T9-006

B4-53005-2T5-M10

B4-23001-2T9-M10

B4-22202-015

B4-22001-003-M10

B4-12001-2T5-M10

B4-44001-2T7-M18

B4-42001-2T7-M18

B4-72002-2T7-G34

B4-72001-2T7-M18

B4-32002-2T3-B03

B2-LS101-2T5-M30

B5-11311-2T3

B5-11001-03-R

B5-13101-003

B5-12101-003

After several years of refinement we are introducing our latest set of improvements all bundled up in one new sensor, the OxyProbe® II. The D545 has a right angled T-Pull connector with D9 cable connector.

The new membrane design is modeled after our successful 25 mm membrane cartridge. It has an expandable inner Silicone bladder that holds the electrolyte within a 316L stainless steel cage. During SIP processes or during autoclaving the electrolyte turns to steam, but does not distend the measurement membrane because the internal bladder expands first, taking the pressure off the sensitive measurement area. This allows us to have a maximum of electrolyte present, with minimum risk of damage to the sensor. The new membrane cartridge design is easy to use and durable enough to be used many times over if necessary.

The D545 (12mm) Oxyprobe II dissolved oxygen sensor uses our standard D9 connector in a right angled design which allows for easy removal from the fermenter and reduces the strain on the cable. It has hermetically sealed gold-plated contacts in a glass insulator, offering the ultimate in signal isolation and transmission. For customers with installed VarioPin cable systems we also offer the D545-VP with VarioPin options available as standard for all of our sensor designs. The D540 DO Sensor offers the same functionality as the D545 but has a straight connector rather than a right-angled T-Pull connector.

Hygienic Sensor Design

  • Micro-organisms cannot adhere to the sensor surface due to the enhanced finish (N5/Ra15µ in Quality).
  • The sensor is fully sterilizable and autoclavable.

Time-Saving Maintenance

  • Membrane body and sensor inner body can be changed out very quickly. A simple membrane body replacement takes just two minutes and can be made on site by the user.
  • Due to the modular design, the different sensors have basically the same spare parts. This minimizes inventory stocking requirements.

D545 Oxyprobe II Dissolved Oxygen Sensor Features Overview

  • Autoclavable and steam sterilizable.
  • Accurate measurement and quick response.
  • Long lasting and easy to maintain membranes.
  • FDA positive listed materials of construction.
  • Hygienically polished N5 surface finish (Max. Ra15µ in).
  • Wetted O-rings comply with FDA and USP Class VI standards.

Other Highlights

  • Comes with either waterproof VarioPin or D9 connector.
  • T-Pull connector versions such as this D545 allow for easy removal from the fermenter and reduce strain on the cable.
  • Variety of sensor lengths available.

Replaceable Anode/Cathode Assembly

A concept pioneered by Broadley-James, this unique assembly permits the sensor to be repaired quickly and reliably. Send your sensors to us for rebuild and save about half the price of purchasing a complete new sensor assembly!

How to choose a sensor

  • Determine how far the sensor should project into the vessel.
  • Decide whether a right angle connector is preferred or a straight connector.
  • Choose the correct cable length and connector. All DO sensors require cable and connector assemblies.

It is important to regularly maintain the sensors by inspecting and calibrating them. The membrane cartridge (see Accessories tab for relevant membrane cartridge kits) should be checked and refilled prior to every use.

12 mm OxyProbe Sensors T-Pull

D9 Sensor Part Number Variopin Sensor Part Number
120 mm D545-B120-PT-D9 D545-B120-PT-VP
220 mm D545-B220-PT-D9 D545-B220-PT-VP
320 mm D545-B320-PT-D9 D545-B320-PT-VP
420 mm D545-B420-PT-D9 D545-B420-PT-VP

Membrane Kits

Broadley-James offer a range of membrane kits to suit all our dissolved oxygen sensors. These include everything required to replace the membrane cartridge and internal o-rings. All kits include:

  • QC Certificate
  • Material Cert

Use our Membrane Replacement instructions for detailed information about replacing the membrane in the D545 (12mm) OxyProbe II Dissolved Oxygen Sensor.

The following membrane kits are suitable for use with the D545 (12mm) OxyProbe II DO Sensor:

Membrane Kit Example Image Kit Contents
Single Cartridge Kit for OxyProbe II

Part No. MK29-01

Single cartridge kit for 12mm DO Sensors One 25 ml bottle of electrolyte

One membrane cartridge

One set of internal o-rings

Four Cartridge Kit for OxyProbe II

Part No. MK29-04

Four cartridge kit for 12mm DO Sensors One 25 ml bottle of electrolyte

Four membrane cartridges

Four sets of internal o-rings

16 Piece Cartridge Kit for OxyProbe II

Part No. MS29-16

 25 Piece cartridge kit for 12mm DO Sensors 16 membrane cartridges in one easy-to-store box.

Internal o-rings and electrolyte must be ordered separately.

OxyProbe II Replacement Cap sleeve

Replacement cap sleeves are available for OxyProbe II Sensors in both standard (Part No. AM-9657-S) and guarded (Part No. AM-9657-G) formats.

Compatible Cables

The D545 T-Pull Autoclavable DO Sensor uses a D4 connector. This industry standard connector was the first, and is still, the best choice for DO sensors. We recommend the following cables for use with the D545 Autoclavable OxyProbe II Dissolved Oxygen sensor:

Cable Type Sensor connector & Device Connector Device Terminal
D4 plug to Lemo 6 D4 plug to Lemo Lemo jack
DO Cable D4 to Crimped Ferrules DO Cable D4 to Crimped Ferrules terminal block

Additional information

Application

Bioprocess

Cap / Connector

D4, Pg13.5, T-Pull

Connection Type / Size

Pg13.5

Features

Autoclavable, RTD, Steam Sterilizable

Installation

In-Line

Measurement

Dissolved Oxygen

Regulatory

FDA Compliant

Size / Type

12 mm

The internal seals of an Oxyprobe are all mechanical seals using o-rings. There are no adhesives to leak or fail in process, making this a more rugged and reliable sensor. Replacing the cathode is also much easier and faster since there is no sealant to clean from the threads. No sealant also makes the job of replacing the cathode much faster and cleaner.