Understanding the Broadley-James Dissolved Oxygen Sensors Range

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Dissolved Oxygen Sensor DesignDO cut away view

Suitable for hygienic applications, the OxyProbe dissolved oxygen sensor withstands Steam in Place (SIP) and caustic Clean in Place (CIP) applications. FDA compliant o-rings on all product contact surfaces.

The outer silicone layer protects the inner Teflon layer, and an added stainless steel mesh provides the mechanical strength required to withstand repeated autoclaving or steam sterilization. The strongest, most robust membrane in the industry, it is an improvement of the industry-standard design. Compatible with existing sensors, there is no need to change sensors or electronics to get improved performance!

Replaceable Anode/Cathode Assembly

A concept pioneered by Broadley-James, this unique assembly permits the sensor to be repaired quickly, easily, and reliably in just a few minutes. A time-proven approach with years of success under the harshest environmental conditions.

FDA Positive Listed Materials of Construction

Electropolished stainless steel surfaces provide better surface cleanability than mechanically polished 16RA alternatives. Copies of 3.1 certificates and mill certificates are available upon request. Every sensor is serialized with paperwork on file in our database.

Universally Compatible

All Broadley-James D.O. sensors work with existing biotech equipment. No proprietary electronics or cables are required. All designs are intended to fit both existing and future requirements, without introducing any unnecessary changes in hardware or cabling. Reduces inventory and confusion, increases flexibility and cost savings.

The Market Leader in D.O. Sensor Design

Unlike other manufacturers, Broadley-James does not believe in changing the basic design every few years forcing customers into buying new equipment to keep current. Instead, Broadley-James strives to make improvements “backwards compatible” so they can be used with new electronics as well as the old.

No changing of a connector which would require new cabling to be run throughout the suite, no special RTDs that would require new transmitters, no changing of cartridge design that forces the customer to obsolete perfectly good, but no longer supported, sensor designs. Instead, Broadley-James strives to protect investments, and help leverage them into the future.

Performance specifications

 Measurement PrincipleAmperometric – Polarographic
 Output signal in AirApprox. 67 nA at 25˚C
 Temperature RangeOperation: 0 to 80˚C
Sterilization: Max. 135˚C
 StabilityBetter than 2% of readout per week at constant pressure and temp. (30˚C)
 Maximum Pressure4 bar (atm.), 58 psig

What makes OxyProbe® sensors unique?

The internal seals of an OxyProbe are all mechanical seals using o-rings. There are no adhesives to leak or fail in the process, making this a more rugged and reliable sensor. Replacing the cathode is also much easier and faster since there is no sealant to clean from the integral threads. Additionally, no sealant makes the job of replacing the cathode much easier and cleaner.


OxyProbe® D.O. Sensors for Standard 25 mm Side PortsModel D400 OxyProbe® D.O. Sensor Series

The OxyProbe D400 and OxyProbe D405 D.O sensors offer the most rugged design for 25 mm side ports. They fit directly into the vessel, eliminating the need for a housing, reducing contamination risks.

The modular design allows quick replacement of any part, making it easy to maintain and repair the sensor. OxyProbe dissolved oxygen sensors carry a 1-year warranty.

Features:

  • 316L Stainless Steel
  • Serialized for traceability
  • RA32 finish with electropolish on wetted parts
  • FDA compliant silicone o-rings
  • Secured retainer ring

OxyProbe®D.O. Sensors for B. Braun Biotech 25 mm Safety Side Ports

Model D430 Series OxyProbe® D.O. SensorsModel D430 Series OxyProbe® D.O. Sensors

The ports of B. Braun Biotech fermentation vessels are longer and have a slightly different internal design. In order to seal properly, the o-ring on models D430 and D435 sensors have been moved closer to the tip of the sensor.

The sensor fits directly into the vessel, eliminating the need for a housing, reducing contamination risks. Few accessories are required. The modular design allows quick replacement of any part, making it easy to maintain and repair the sensor. OxyProbe dissolved oxygen sensors carry a 1 -year warranty

Features:

  • 316L Stainless Steel
  • Serialized for traceability
  • RA32 finish with electropolish on wetted parts
  • FDA compliant external EPDM o-ring
  • Secured retainer ring

Broadley-James® Smart Polarizer – Conditions a Polarographic D.O. Sensor AM-9660

Smart Polarizer

The Broadley-James Smart Polarizer conditions a polarographic dissolved oxygen sensor, so that it is ready to use and also allows the user to test the sensor to ensure it is operating correctly before it is placed in the vessel.

When the dissolved oxygen sensor is connected to the Smart Polarizer a polarizing voltage is automatically applied to the sensor. The unit displays the raw nA output from the sensor, the temperature from the sensor’s internal temperature compensator and the unit’s battery voltage.

This allows the user to test the dissolved oxygen sensor raw output (nA) in air (100%) and in zero oxygen conditions (0%).

Benefits:

  • Full zero and air test to verify the dissolved oxygen sensor is fit for purpose
  • Prevents a faulty sensor being fitted to a vessel
  • Simplifies testing procedures
  • Reduces operational costs

Features:

  • Convenient: One unit to test and polarize
  • Universal: Can be used with all popular makes of polarographic sensors
  • Battery Powered: Can be used in the lab or production areas
  • Portable: Small pocket size

OxyProbe®19 mm D.O. Sensors for 19 mm Top Ports

Model D200 Series OxyProbe® sensorsModel D210 Series OxyProbe®D.O. Sensors

The OxyProbe D200 and D205 19 mm dissolved oxygen sensors feature the same rugged and versatile design as the 25 mm OxyProbe. Although the 19 mm OxyProbe sensor has a slightly smaller diameter than the 25 mm sensor, it utilizes the same anode/cathode assembly and membrane cartridges.

The 19 mm OxyProbe is often used in both process and benchtop applications, which are equipped with special 19 mm top ports The Model D200 series dissolved oxygen sensors are made of 316L stainless steel to withstand the bioprocess environment.

Each sensor is serialized for traceability, and the port retainer ring is secured to the sensor to prevent loss. All Broadley-James dissolved oxygen sensors carry a 1-year warranty.


OxyProbe® D.O. Sensor with 1.5” Sanitary Flange for Sanitary Pipe Tee and Novaseptic Side Ports

Model D210 Series OxyProbe®D.O. SensorsModel D210 Series OxyProbe®D.O. Sensors

The OxyProbe D210 and OxyProbe D215 D.O sensors are unique in their construction. The front end of the sensors are the same as the 19 mm sensor, with the smaller diameter and no crevices or o-rings. They fit directly into a 1.5” sanitary pipe tee, eliminating the need for a housing, reducing contamination risks. Designed with an integral 1.5” sanitary flange, a gasket seal at the flange holds the sensor securely in place.

It is important to note, these sensors are not intended for use with the standard 25 mm sanitary flange ports. However, they can be used with the NovAseptic ports.

The interior design and replacement parts of these sensors are the same as other 25 mm OxyProbes. The modular design allows quick replacement of any part, making it easy to maintain and repair the sensor. OxyProbe dissolved oxygen sensors carry a 1-year warranty.

Features:

  • 316L Stainless Steel
  • Serialized for traceability
  • RA32 finish with electropolish on wetted parts

Additional Features Include:

  • Fits in both sanitary flange ports and NovAseptic ports
  • Can also be used in-line in a sanitary pipe tee

It is important to maintain the sensors by inspecting and calibrating them regularly. The membrane cartridge should be checked and refilled prior to every use.

All OxyProbe dissolved oxygen sensors are available with a right-angle connector. This low profile design provides:

  • Protection against cable fatigue
  • Improved grip for sensor extraction
  • Reduced random sensor mobility on flat surfaces (won’t roll off the table)

OxyProbe® 12 mm D.O. Sensors for Benchtop Vessels

Model D140 Series OxyProbe® D.O. SensorsModel D140 Series OxyProbe® D.O. Sensors

The OxyProbe D140 and OxyProbe D145 D.O sensors offer the most rugged and versatile design for benchtop vessels. Their compact profile saves space on a crowded head plate. They will fit directly into any 12 mm multipurpose port with standard compression fittings or Pg13.5 threads. The small diameter facilitates D.O. measurement in small volumes, especially bottles and spinner flasks.

These sensors have the same rugged performance as the 19mm and 25mm sensors. These polarographic sensors utilize the longer silver anode and small cathode which has become the industry standard. They also use the same gas-permeable, silicon/Teflon composite membrane, reinforced with stainless steel mesh.

All OxyProbe dissolved oxygen sensors are available with a right-angle connector. This low profile design provides:

  • Protection against cable fatigue
  • Improved grip for sensor extraction
  • Increased clearance between vessels
  • Reduced random sensor mobility on flat surfaces (won’t roll off the table)

It is important to regularly maintain the sensors by inspecting and calibrating them. The membrane cartridge should be checked and refilled prior to every use.

Take a look at our FAQ page for more information on selecting sensors for benchtop vessels.