3 Liter Bioreactor Assembly (Cell Culture)

The following blind stoppers are available for the (M18 x 1.5 mm or G-3/4) threaded headplate ports and include Silicone O-ring.

O-ring Sizes:

G-3/4 = AS-023 Silicone

Compatible with 2 – 7 liter vessel, G3/4 port.

The following blind stoppers are available for the (M18 x 1.5 mm or G-3/4) threaded headplate ports and include Silicone O-ring.

O-ring Sizes:

M18 =  20 x 2.0

Compatible with 2 – 7 liter vessel, M18 x 1.5 port.

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring sizes:

12 mm = AS-111

Compatible with 2 – 20 liter vessel, 12 mm port

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring size:

10 mm = 8.5 x 2.5

Compatible with 2 – 20 liter vessel, 10mm port.

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring size:

6 mm =   AS-107

Compatible with 2 and 3 liter vessel, 6 mm port.

These are the fluted nuts used to attach the vessel headplate to the vessel base. Sold in packs of six each, sized for the 2 – 20 liter vessels.

Compatible with 2 – 20 liter Vessel base.

This height adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes, and a 6 mm OD x 4 mm ID sample tube. With this device, the culture fluid can be sampled at any desired level. Includes 10 mm Hex Nut Washer and 8.5 x 2.5 mm Silicone O-ring.

Maximum insertion length:          213 mm (2 – 5 liter reactor)

Compatible with 2 – 5 liter vessel, 10 mm port.

The air overlay pipe can be used for either gas outlet or gas overlay. The latter use is for headspace aeration (separate from or in combination with sparging gas through the culture). Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 2-20 liter Vessels, 10 mm port.

Attaches to the tip of the Sparger Tube, this 316L stainless steel sintered tip is available in a variety of pore sizes. The 15 Micron pore frit is the standard size supplied with the sparger tube. Other sizes available upon request (see table). Includes 5.7 x 1.9 mm Silicone O-ring.

Air inlet pipe used to meet the oxygen demand of a culture. Allows a sterile gas stream to be sparged into the vessel. This is a “sintered frit” or “stone” type sparger which can be used when high gas flows might cause shear damage. The 316L sintered bead porous sparger generates very small bubbles, resulting in very efficient gas transfer. Supplied with 15 Micron sparge tip. Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 3 liter vessel, 10 mm port.

The thermowell allows you to insert a temperature probe in the reactor. Fill the thermometer pocket with water or silicone oil in order to improve thermal contact between the culture and the probe. Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 2 – 5 liter vessel, 10 mm port.

Insertion length: 200 mm (for 2 – 5 liter reactor)

The septum holder is equipped with a silicone rubber septum and can be used as a universal addition port by piercing it with one or more needles. Includes 20 x 2.0 mm Silicone O-ring.

Compatible with 2 – 7 liter vessels, 18 mm port.

The media inlet triple port allows you to equip one M18 x 1.5 mm threaded port with three addition ports (e.g. for acid, alkali and anti-foam addition). This device can be used to expand the number of entries beyond the number of ports in the headplate. Includes 20 x 2.0 mm Silicone O-ring.

Compatible with 2-7 liter Vessels, M18 x 1.5 port.

This housing fits into a G-3/4” threaded port and can accommodate a pH or ORP electrode, a Dissolved Oxygen sensor, a Cell Density probe, or other 12 mm OD tubes. All items that are held by this housing are at a fixed depth. Includes AS-111 internal and AS-023 external Silicone O-rings.

Compatible with 2 – 7 liter vessel, G3/4” port.

This housing fits into an M18 x 1.5 mm threaded port and can accommodate a pH or ORP electrode, a Dissolved Oxygen sensor, a Cell Density probe, or other 12 mm OD tubes. All items that are held by this housing are at a fixed depth. Includes AS-111 internal and 20 x 2.0 mm external Silicone O-rings.

Compatible with 2 – 7 liter vessel, M18 x 1.5 port.

The impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Vortex Pitched Blade.

Installs into the central M30 x 1.0 mm port of the 2 through 5 liter vessels. Includes 32 x 2.5 mm Silicone O-ring.

Material: 316L Stainless Steel

Shaft Diameter: 8 mm

Compatible with 2-5 liter Vessel M30 Port

316L stainless steel stand for 3 liter vessels. Includes adjustable braces to stabilize the lower portion of the vessel, and protective silicone rubber feet for the three legs. This prevents wobble and stray signals which may be transferred through the legs when placed in multiples on steel clad benchtops.

Compatible with 3 liter Vessel & Accessories.

Dished bottom bioreactor vessel, suitable for both fermentation and cell culture runs. Dished bottom ensures optimum mixing. Marked with approximate volume graduations, made from borosilicate glass, fully autoclavable.

Compatible with 3 liter vessel stand and single piece retainer ring.

Connects to the 3 liter vessel, forming a seal between the vessel and the stand. Single piece, 316L stainless steel.

Includes:

• 6 each M6 x 45 mm screws (attached)
• 3 each M4 x 16 mm flat head screws (ring to stand)
• 1 each Teflon/Silicone Gasket
• 1 each 150 x 4.0 mm Silicone O-ring

Comptible with 3 liter Vessel & Accessories

316L stainless steel electropolished headplate for 3 liter vessels. Threaded ports for a wide variety of accessory fittings. O-rings, fittings, etc. purchased separately.

Compatible with 3 liter Vessel & Accessories.

Kit to self-rebuild the Lipseal Agitator Assembly.

Compatible with 2-20 liter Small Agitator Assembly.

This height adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes (B4-71002-2T9-M10), and

a 6 mm OD x 0.78 mm ID sample tube.

This sample pipe has a very small dead volume. The height adjustable sample pipe can be used with or without a sample screen. If it is used without a sample screen, the small dead volume of the pipe guarantees samples from the culture that are representative for the reactor.

If a sample screen is used at the end of this pipe, cell-free samples can be drawn from the culture. Sample screens are available in several pore sizes.

Compatible with 2 – 5 liter vessel, 10 mm port.

Installs into the central M30 x 1.0 mm threaded port of the 2 through 5 liter vessels. Includes 32 x 2.5 mm Silicone O-ring.

Material: 316L Stainless Steel

Shaft Diameter: 8 mm

Compatible with 2 – 5 liter vessel, M30 port

This device fits into the standard M18 x 1.5 threaded port and allows for cooling water to be circulated through it, chilling the contents of the vessel. Standard 6 mm tube fittings for attaching the cold-water tubing can be hooked up to either a standard bench water supply or to a recirculating chiller pump. Includes 20 x 2 mm Silicone O-ring.

Compatible with 2 – 5 liter vessel, 18 mm port.

The following impeller is available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, includes size M3 x 5 mm hex set-screw.

Compatible with 2 – 3 liter vessel, 8 mm shaft.

Vessel operation at elevated temperature combined with aeration of the culture might cause too much evaporation during a run (increasing the nutrient concentration and decreasing volume); this can be prevented by using an air-outlet condenser. Includes Teflon spacer AS-111 Silicone O-ring and B472001-2T7-M18 Headplate fitting shown.

Standard 6 mm tube fittings for attaching the cold water tubing can be hooked up to either a standard bench water supply or to a recirculating chiller pump.

Compatible with 2 – 5 liter vessel, M18 x 1.5 port Sensor Fitting.

The impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Scoping Pitched Blade.