20 Liter Bioreactor Assembly (Cell Culture)

Installs into the central M69 port, which has been adapted to accept a M30 size agitator. Typical for cell culture applications.

Material: 316 L Stainless steel

Shaft diameter: 8mm

Compatible with 20 liter Vessel, M30 port

Requires port adaptor plate: B2–AD105–8T9–M69

This height-adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes, and a 6 mm OD x 4 mm ID sample tube. With this device, the culture fluid can be sampled at any desired level. Includes 10 mm Hex Nut Washer and 8.5 x 2.5 mm Silicone O-ring.

Insertion length is 553 mm (20 liter reactor).

Compatible with 20 liter Vessel, 10mm Port.

Air inlet pipe used to meet the oxygen demand of a culture. Allows a sterile gas stream to be sparged into the vessel. This is a “sintered frit” or “stone” type sparger which can be used when high gas flows might cause shear damage. The 316L sintered bead porous sparger generates very small bubbles, resulting in very efficient gas transfer. Supplied with 15 Micron sparge tip. Includes 10mm Hex Nut, Washer and 8.5 x 2.5mm Silicone O-ring.

Compatible with 20 liter Vessel, 10mm port.

The thermowell allows you to insert a temperature probe in the reactor. Fill the thermometer pocket with water or silicone oil in order to improve thermal contact between the culture and the probe.

Insertion length:           552 mm (for 20 liter reactor)

Compatible with 20 liter Vessel, 10mm port.

316L stainless steel stand for 20 liter vessels. Includes adjustable braces to stabilize the lower portion of the vessel, and protective silicone rubber feet for the three legs. This prevents wobble and stray signals which may be transferred through the legs when placed in multiples on steel clad benchtops.

Compatible with 20 liter Vessel & Accessories.

Dished bottom bioreactor vessel, suitable for both fermentation and cell culture runs. Dished bottom ensures optimum mixing. Marked with approximate volume graduations, made from borosilicate glass, fully autoclavable.

Compatible with 20 liter Vessel, Stand & Accessories.

The following blind stoppers are available for the 27mm straight bore headplate ports. Includes 27 mm Hex Nut, Washer and AS-121 Silicone O-ring.

Compatible with 15 & 20 liter Vessels, M27 port.

The septum holder is equipped with a silicone rubber septum and can be used as a universal addition port by piercing it with one or more needles. Includes 27mm Hex Nut, Washer and AS-121 Silicone O-ring.

Compatible with 15 & 20 liter Vessels, M27 Port.

The medium inlet triple port allows you to equip one standard 27mm port with three addition ports (e.g. for acid, alkali and anti-foam addition). This device can be used to expand the number of entries beyond the number of ports in the headplate.

Includes 27mm Hex Nut, Washer and AS-121 Silicone O-ring.

Compatible with 15 & 20 liter Vessels, M27 port.

This housing fits into an M27 port and can accommodate a pH or ORP electrode, a Dissolved Oxygen sensor, a Cell Density probe, or other 12 mm (o.d.) tubes. All items that are held by this housing are at a fixed depth. Internal thread is pg13.5. Includes 27 mm Hex Nut, Washer and AS-121 Silicone O-ring.

Compatible with 15 & 20 liter Vessels, M27 port.

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Vortex Pitched Blade 15mm.

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Vortex Pitched Blade 8mm.

Connects to the 15 and 20 liter vessels, forming a seal between the vessel and the stand. Single piece, 316L stainless steel.

Includes:

• 6 each M6 x 53 mm screws (attached)
• 3 each M4 x 16 mm flat head screws (ring to stand)
• 1 each Teflon/Silicone Gasket
• 1 each AS-274 Silicone O-ring

Compatible with 15 & 20 liter Vessels, Stands & Accessories.

316L stainless steel electropolished headplate for 15 and 20 liter vessels. Threaded ports for a wide variety of accessory fittings. O-rings, fittings, etc. purchased separately.

Compatible with 15 & 20 liter Vessels & Accessories.

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring sizes:

12 mm = AS-111

Compatible with 2 – 20 liter vessel, 12 mm port

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring size:

10 mm = 8.5 x 2.5

Compatible with 2 – 20 liter vessel, 10mm port.

These are the fluted nuts used to attach the vessel headplate to the vessel base. Sold in packs of six each, sized for the 2 – 20 liter vessels.

Compatible with 2 – 20 liter Vessel base.

The air overlay pipe can be used for either gas outlet or gas overlay. The latter use is for headspace aeration (separate from or in combination with sparging gas through the culture). Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 2-20 liter Vessels, 10 mm port.

Attaches to the tip of the Sparger Tube, this 316L stainless steel sintered tip is available in a variety of pore sizes. The 15 Micron pore frit is the standard size supplied with the sparger tube. Other sizes available upon request (see table). Includes 5.7 x 1.9 mm Silicone O-ring.

This device fits into two M10 ports and allows for cooling water to be circulated through it, chilling the contents of the vessel. Standard 6mm tube fittings for attaching the cold water tubing, can be hooked up to either a standard bench water supply or to a re-circulating chiller pump. Includes 2 each 10 mm Hex Nuts, Washers and 8.5 x 2.5 mm Silicone O-rings.

Compatible with 20 liter Vessel, 10mm ports.

This is used for heating the vessel contents and controlling to a set value, say 37 degrees Centigrade. Made of flexible silicone coated heating elements, it plugs into a standard 110 VAC temperature controller or vessel control system.

Standard 6’ cord length (Special lengths up to 15’ available on request).

Compatible with Standard 110 VAC Temp Controller.

The following impeller is available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, includes size M3 x 5 mm hex set-screw.

Compatible with 15 & 20 liter Vessels, 15mm shaft.

The following impeller is available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, includes size M3 x 5 mm hex set-screw.

Compatible with 15-20 liter Vessel, 8mm shaft.

Vessel operation at elevated temperature combined with aeration of the culture might cause too much evaporation during a run (increasing the nutrient concentration and decreasing volume); this can be prevented by using an air-outlet condenser.

Includes Teflon spacer, AS-111 Silicone O-ring and B4-72004-8T9-M27 Headplate fitting shown.

Standard 6 mm tube fittings for attaching the cold water tubing can be hooked up to either a standard bench water supply or to a recirculating chiller pump.

Compatible with 15 & 20 liter Vessels, M27 x 1.5 Port.

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Scoping Pitched Blade 15mm.

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm or 15 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Compatible with Scoping Pitched Blade 8mm.

Compatible with 15 & 20 liter Large Agitator Assembly.

This height adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes (B4-71002-2T9-M10), and a 6 mm OD x 0.78 mm ID sample tube.

This sample pipe has a very small dead volume. The height adjustable sample pipe can be used with or without a sample screen. If it is used without a sample screen, the small dead volume of the pipe guarantees samples from the culture that are representative for the reactor.

If a sample screen is used at the end of this pipe, cell-free samples can be drawn from the culture. Sample screens are available in several pore sizes.

Compatible with 7-20liter Vessel, 10mm Port.